Introduction of MePhe 12 imposed steric restraints that hindered synthesis, which we reduced by replacing Thr 11 with serine
OpenSource Diets formula D12451) or, if they were previously on an LD, they were maintained continued on LD (termed lean control mice)
Pay particular attention to the forms of nutrients included: methylcobalamin or cyanocobalamin for B12, cholecalciferol (vitamin D3) rather than ergocalciferol (D2), and minerals in forms that support good absorption
Tesofensine operates through a completely different mechanism: it blocks reuptake of dopamine, norepinephrine, and serotonin simultaneously, creating a triple monoamine effect that intensifies with every compound that touches those same pathways
Most successful programs incorporate monthly check-ins to monitor progress, adjust dosing if needed, address any side effects, and maintain accountability