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The ability of a small peptide to reproduce aspects of dual incretin receptor activation in vitro, underscores its potential relevance as a lead scaffold

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Briefly, an XhoI/EcoRI fragment containing a U6-shRNA cassette was amplified from pLKO.1 vector using high-fidelity PCR and ligated into XhoI/EcoRI-digested adenoviral shuttle vector pacAd5 K-NpA

Interest in this possibility was rekindled by John Dupr, a Canadian endocrinologist at St Thomas' Hospital in London