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TKT has two active centres with one THDP molecule attached to a binding motif [98, 99] and a bivalent cation (Ca affinity more than Mg [100]) tightly bound at each centre by noncovalent interactions [101]

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Rescue at the DNA Level Gene therapy encompasses techniques that can alter the expression of an organisms genes by targeting the DNA either through transgene delivery, or by direct modification of the genome, with the goal of therapeutically restoring a pathologically expressed gene to normal expression levels [38]

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