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Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) assay Plasma AST and ALT were measured using BioAssay Systems EnzyChrom Alanine Transaminase Assay Kit (cat# EALT-100) and EnzyChrom Aspartate Transaminase Assay Kit (cat# EASTR-100) according to the manufacturers instructions