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Then, 5 L of the supernatant was injected into the LC-MS system (A), with separate injections performed for both positive and negative ionization modes

Moreover, in comparison to normal esophageal epithelium, ZNF282 has been frequently reported to be overexpressed in ESCC tissues and ZNF282 depletion increased apoptosis and promoted cell cycle arrest at G1/S, suggesting that ZNF282 transcriptional co-activator, plays pivotal role in controlling E2F1-mediated ESCC progression