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The following conditions were used for the thermal cycler (T100 Thermal Cycler, BIO-RAD, Hercules, CA, USA): 10 min at 25 C, 120 min at 37 C, 5 min at 85 C, and 10 min at 4 C
GHK, CuCl 2 and Cu(OAc) 2 were used at a final concentration of 58 M and 580 M
The combination provides both immediate availability and sustained production of NAD+ [11]
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In this context, various biological options have arisen to alleviate pain and enhance functional abilities
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