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Following post-hybridization stringency wash steps, sections were treated with 0.5% Triton X-100/0.5% H 2 O 2 in PBS for 15 min, rinsed in PBS, immersed in maleate buffer (0.1 M maleic acid, 0.15 M NaCl, pH 7.5

S2CID 19304098

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The antibody Fab7F38 appears to function by providing enhanced soluble surface area for crystal lattice packing, and indeed Fab7F38-bound GLP-1R can also assume an active conformation as shown by EM studies (see below), suggesting that Fab7F38 does not interfere with the conformational flexibility of the ECD during crystallization

Despite limited data on the interaction between integrins and OA FLS, differences in integrin expression were observed between the lining and sublining layers of the synovium