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Total cellular protein was extracted in lysis buffer and protein levels were quantified using a BCA (bicinchoninic acid) Protein Assay kit (Beyotime Biotechnology, Haimen, China)

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Embryonic Wnt/-catenin signaling decreases long-term potential of hair follicle stem cells Given that attenuated Wnt/-catenin signaling is required for HFSC specification, it was puzzling to observe that a small portion of HFSCs originated from Axin2+ hair peg cells (Figure 4A,B)

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