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Add 3.0 mL bacteriostatic water to one 1 mg vial ~0.333 mg/mL (333 mcg/mL), a large dilution chosen for accurate low-dose measuring

As with shoulder injections, make a triangle with the thumb and forefinger

Pregnant or breastfeeding women: Supplement safety in these groups is not fully established

PMID 32571982

Published research describes the following mechanisms for MC1R agonists like afamelanotide: NF-kappaB suppression reduced transcription of pro-inflammatory genes Cytokine modulation decreased TNF-alpha, IL-1beta, and IL-6 production Nitric oxide regulation normalization of NO pathway signaling Adhesion molecule downregulation reduced inflammatory cell migration IL-10 upregulation increased anti-inflammatory cytokine output These effects have been demonstrated in vitro and in animal models of acute, chronic, and systemic inflammation (Catania et al., 2004)