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After 48 h incubation at 27C, the cells were collected by centrifugation and stored at 80C until use

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3gss: AJ Oakley, J Rossjohn, M Lo Bello, AM Caccuri, G Federici & MW Parker (1997) The three-dimensional structure of the human Pi class glutathione transferase P1-1 in complex with the inhibitor ethacrynic acid and its glutathione conjugate