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However, these approaches increase injection burden, multiply opportunities for measurement error, and deviate even further from studied protocols

As previously described 21 , four microliters (4 g) of reconstituted peptides were loaded and washed on a Pepmap100 C18 trap (5 m, 0.3 5.0 mm, Thermo Fisher Scientific, Waltham, MA, USA) at a flow rate of 20 L/min

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