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Off-Label Use This is a major point of confusion

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EC50 values obtained using this method were then used to calculate: sample ratio = sample EC50/EC50 reference peptide-spiked plasma sample and estimated concentration = Known top concentration of peptide- spiked plasma sample/sample ratio

These experimental observations can be well described by a phase diagram that predicts a phase transition between liquid and liquid-crystal phases under near-physiological conditions due to the presence of cholesterol esters (Shimobayashi and Ohsaki, 2019)