ELF GSH levels have been shown to be inversely correlated with proinflammatory cytokine release both in vitro and in vivo [16, 34]
Additionally, these injections can alleviate the incapacitating symptoms linked to such migraines
To obtain ratiometric images, the masks were applied to the background-subtracted images and the FP channel was divided by the SiR channel in a pixel-by-pixel manner within the ROIs
Tests involving multiple factors were analyzed using either a three-way analysis of variance (ANOVA) with diet, time, and sex as variables, a two-way ANOVA with diet and time as variables, followed by Sidaks post hoc test, a two-way repeated measures (RM) ANOVA for longitudinal data, followed by Dunnetts post hoc test or by one-way ANOVA followed by Tukeys post hoc test as specified in the figure legends
Using a 23G needle will cause unnecessary pain, increase the risk of tissue trauma, and likely lead to bruising and minor bleeding at the injection site