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The apparent permeability coefficients (P app ) were calculated as described previously [40] with the following equation: where [C] A is the concentration difference of in the acceptor compartment at 24 h, [C] D is the concentration in the donor compartment at 0 h, V A is the volume of the acceptor compartment (0.9 ml), and A is the surface area available for permeability (0.33 cm 2 )

2F, the changes in the absorbance max peak wavelength as a function of the denaturation times are displayed

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