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In the myocardial injury model, cardiac fibroblast-derived Exos inhibit SLC7A11 expression and stimulate cardiac ferroptosis by delivering miR-23a-3p [223]

Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

Similarly, albeit also in a small cohort, Comhair et al

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With static mixers, the fluid moves through the mixer, rather than some part of the mixer, such as a blade, moving through the fluid