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After washing with PBS, samples were incubated with Alexa Fluor 488- or 555-conjugated secondary antibodies (Invitrogen, California, A-21202/A-31572, USA) for 1 h at room temperature in the dark and stained with 4,6-diamidino-2-phenylindole (DAPI)
10.1007/s00259-009-1363-y 10 BromM.Woliner-van der WegW.JoostenL.FrielinkC.BouckenoogheT.RijkenP.et al (2014)
Five types of ceramides (EOP, NG, NP, AG, AP) for barrier support and hydration
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