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[DOI] [PMC free article] [PubMed] [Google Scholar] 36.Qu D, Ludwig DS, Gammeltoft S, Piper M, Pelleymounter MA, Cullen MJ, et al

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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)