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2 Materials and methods 2.1 Animal experiment and sampling The study was conducted at the experimental station of the Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), located in Braunschweig, Germany, in full compliance with the German Animal Welfare Act and approved by the Lower Saxony State Office for Consumer Protection and Food Safety (LAVES, Oldenburg, Germany) (AZ33.19-42502-04-16/2378)