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(6) Transwell migration and invasion assays The transwell migration and invasion assays were conducted using a 24-well transwell chamber (Corning, USA) and 8-m pore size membranes (Corning, USA)

The inhibition of NF-B by zinc via A20 and PPARs signaling pathways are the most important mechanism because zinc decreases pro-inflammatory cytokines in atherosclerosis [35]

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