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Quantification of membrane-bound total heme and hemichromes in ghosts prepared from stage-separated infected RBCs Heme bound to the cytoplasmic face of the membrane as hemoglobin and insoluble hemichromes was analyzed in the hypotonic ghosts [64] prepared under non-reducing conditions in the presence of Complete protease inhibitor cocktail from the bottom Percoll fraction of the unseparated, infected GR-deficient and normal RBCs [22]

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