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Inclusion criteria required patients aged 18 years or older with type 2 diabetes mellitus (T2DM), with A1c values less than 10%, and at least one cardiovascular risk factor (CVRF) including hyperlipidemia, essential hypertension, overweight or obesity, and/or chronic ischemic heart disease

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qRT-PCR was carried out on a Hongshi SLAN-96P instrument after adding primers and TB Green Premix Ex Taq (2X) (RR420L, Takara, Japan) to the 10-fold diluted cDNA

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