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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C

Research suggests that women who take part in weight loss programmes before undergoing in vitro fertilisation (IVF) are 47% more likely to get pregnant naturally than those who receive no or minimal weight loss support.

In clinical practice, a person diagnosed with type 2 diabetes who has a normal BMI with no features of metabolic syndrome is a good clue for the possible presence of autoantibodies and, potentially, a LADA diagnosis

doi:10.3389/fneur.2021.751736 Spencer TJ, Brown A, Seidman LJ, et al