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Cytotoxicity was determined using a 3-(4,5-dimethyl 2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay

GLP-1 agonists work through distinct mechanismsenhancing glucose-dependent insulin secretion, suppressing glucagon, delaying gastric emptying, and promoting central satiety signaling

For example, SCFA receptors such as GPCR41 and GPCR43, medium and long-chain fatty acid receptors GPCR40 and GPCR120, and the chenodeoxycholic acid receptor farnesoid X receptor (FXR) expressed in intestinal cells and other tissues have been associated with the modulation of various host-specific metabolic processes, including the inhibition of insulin secretion, reduction of non-esterified fatty acid release, suppression of bile acid synthesis, and regulation of fatty acid metabolism 5

PMID: 102 DonadeuFSanchezJMohammedBIoannidisJStenhouseCMaioliMet al