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Promega) (for AMYRs) or coelenterazine h (Nanolight Technologies, Pinetop, AZ) (for CTR) was then added to cells at a final concentration of 1:1000 or 5 M, respectively, and incubated for a further 1015 min

USP guidance describes a 28-day beyond-use date for multi-dose vials after first puncture, assuming aseptic technique on every draw

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