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Additionally, ER stress can induce AMPK activation via increased cytosolic Ca 2+ levels, providing an alternative route for autophagy initiation (Hyer-Hansen et al., 2007)

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Cell culture studies HEK293T cells (CRL-3216, ATCC) were cultured in DMEM (11995073, Life Technologies) supplemented with 10% heat-inactivated FBS (10500064, Life Technologies), 100 IU ml 1 penicillin and 100 g ml 1 streptomycin solution (Pen/Strep, P4333, Sigma Aldrich)

GLP-1 2 ,