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Cells were cultured in Dulbeccos Modified Eagles medium (Welgene LM001-05) supplemented with 10% fetal bovine serum (Gibco 16000-044), and 1% penicillin/streptomycin (Gibco 15140-122)

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The resultant increased level of ENaC in the membrane results in increased Na + reabsorption with concomitant increased K + efflux via the ROMK channel

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8 SOX-10 is a transcription factor that plays an important role in the development of neural crestderived cells, including Schwann cells and melanocytes