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Specifically, HT22 cells were cultured in 96well plates (1.0 10 4 cells/well) for 24 h and then pretreated with A 142 (25 M) for an additional 24 h to construct neurotoxic models

The animals were housed on a 12-hour light/dark cycle with free access to food and water, and all experiments were performed during the light phase of the cycle

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The catch is that qualification requirements can be strict, and patients on government insurance like Medicare or Medicaid are typically excluded

For many, that can include the need for a weekly subcutaneous injection