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Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells

The animal housed individually in stainless steal cages under controlled condition at constant temperature (22C) and lighting (12 light-dark cycle) and given free access to food and water at all times

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For the flow cytometry assay, 3 10 4 cells/well/40 L were incubated with 5 nM of mono- or bispecific antibody constructs in flow buffer (DPBS w/o Ca and Mg (PAN-Biotech) with 3% heat inactivated FBS (Gibco)) for 1 h at 4 C in a V-Bottom 384 well plate (Greiner)