Good Lab Practices: Use appropriate PPE, follow institutional protocols, and reference your labs chemical hygiene plan
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[DOI] [PMC free article] [PubMed] [Google Scholar] 135.Kim Y., Vadodaria K.C., Lenkei Z., Kato T., Gage F.H., Marchetto M.C., Santos R
2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added