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Alternatively, certain molecules like 2-nitroimidazole have the ability to generate 1 O 2 and create a hypoxia microenvironment when exposed to 619 nm for treatment of MCF7 breast cancer cell lines [150]

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Then, the membrane containing the protein was blocked with TBST buffer containing 5% skim milk powder for 1 h at room temperature

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Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached