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Fourier transform infrared spectroscopy (FTIR) analyses Tested samples of pure LS extract, plain nanospanlastics, and selected formulations of LS-loaded nanospanlastics were analyzed using an FTIR spectrophotometer (Hitachi-Tokyo-Japan) using the potassium bromide disc method

The metal-peptide bond can dissociate at elevated temperatures, releasing free copper and destroying the active complex

Each stimulus lasted 650 ms

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This heat will convert the excess tissue in the process into steam