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In April 2026 the sponsor announced positive topline results from the Phase 3 SYNCHRONIZE-1 obesity trial, but those company-reported data await full peer-reviewed publication and are best treated as preliminary until then

Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST

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In vitro ATP binding assays were performed with recombinant GST-PDK1 WT and K163R proteins purified from E

After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)