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After a 24 h 100 nM incretin-based peptide pre-treatment and a 24 h 1 ng/mL LPS pro-inflammatory stimulus, cells were harvested using a cell scrape, collected by centrifugation, and re-suspended in RIPA buffer (R0278, Sigma-Aldrich) with 1X Halt TM Protease & Phosphatase Single-Use Inhibitor Cocktail (78442, ThermoFisher Scientific, Waltham, MA, USA) for lysis
Testing may be appropriate if: Weight loss exceeds 1520% Fatigue, hair thinning or neuropathy develops Persistent vomiting or diarrhoea occurs Dietary intake becomes severely restricted You are in a higher-risk group (e.g., premenopausal women, older adults, GLP-1 + metformin users) Monitoring should be personalised rather than automatic
J Diabetes Investig 1:823 Samms RJ, Coghlan MP, Sloop KW (2020) How may gip enhance the therapeutic efficacy of GLP-1
Tests in obese mice indicated the triple agonist was more powerful than dual agonists in correcting obesity and diabetes (Finan, Yang, Ottaway)
Including fermented foods regularly in your diet may support gut health, though specific effects on GLP-1 in humans require further research
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