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Forty-eight hours after plating, cells were rinsed with HBSS (Sigma-Aldrich), and cells were further incubated in medium containing 0.5% FBS and 5 mM glucose

We then briefly consider clinically approved GLP1 receptor ligands and recent advances in this field

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The primers pVK-1fw (5GGGGATATCCACCATGGAGACAGACACACTCCTGCTAT 3) and pCK-2rev were used for the amplification

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