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Parasite cultures were centrifuged, the pellets resuspended with 20 volumes of saponin lysis buffer (0.02% saponin, 10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 145 mM NaCl, 3 mM KCL, pH 7.2), incubated for 10 min at 37C, and washed three times with phosphate-buffered saline

Sevuparin is a novel heparin-derived compound that retains selectin-binding abilities but lacks the anticoagulant qualities of heparins [137]

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Peer review Peer review information Nature Communications thanks Joseph Gordon, Yong-ho Lee and the other, anonymous, reviewer(s) for their contribution to the peer review of this work