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Apart from differential compartmentation, one possibility is that this array of enzymes guarantees robust redox homeostasis, even at the expense of redundancy, consistent with loss-of-function studies for APX and PRX isoforms in the chloroplast (Awad et al

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Folate, vitamin B6, and vitamin B12 are all required for the conversion of homocysteine into the sulfur-containing amino acid methionine

Natural glutathione production is easily disrupted however, and stores can become quickly depleted by the ravages of poor diet, stress, medications, infections and radiation and other toxins

The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)