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Recent approaches integrating chemogenetic modulation (e.g., clozapine-N-oxide induction) with fluorescence-activated cell sorting and transcriptomic profiling have enabled detailed mapping of activity-dependent gene networks in endothelial cells, key BBB transporters such as P-glycoprotein show rhythmic expression coordinated with core clock genes, generating time windows that favor CNS drug penetration (54, 55)

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