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However, some optimistic signs are dawning, with some recent works employing cutting-edge technologies such as patient-derived organoid culture and single-cell RNA sequencing, which have connected ASD development with the haploinsufficiency of epigenetic factors and chromatin remodelers (SUV420H1/KMT5B, ARID1B, and CHD8) [196], while the inhibition of LSD1 demethylase has been observed to reverse ASD phenotypes in mice [197]