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After 30 min incubation at room temperature, the treated lysates were further divided into smaller aliquots (50 l), which were then heated at different temperatures for 3 min on a Veriti thermal cycler (Applied Biosystems) followed by cooling for 3 min to room temperature

If a serum arrives already tinted brown or smells off, that can indicate oxidation

Han X, Chang L, Qiu Z, Lin M, Wang Y, Liu D

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Midbrain dopamine oxidation links ubiquitination of glutathione peroxidase 4 to ferroptosis of dopaminergic neurons