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Real-time RT-PCR Livers were isolated and total RNA was extracted and purified using the RNeasy kit (Qiagen, Hilden, Germany)

Rosette formation in chicks: with special reference to QRBC-rosettes

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Efficiacy results were analysed based on the reported efficacy estimand (calculated from data gathered while patients received their assigned randomized treatment, excluding data after discontinuing the allotted medication or after adding rescue medication