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Description

Cozzoli, D

Drawing from a nearly empty vial As your vial empties, the remaining liquid pools at the bottom and around the rubber stopper

doi: 10.1083/jcb.9.2.493

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After washing once with PBS to remove unbound CFSE, SFN was added for 30 min and the cells were seeded on 96-well microplates coated with anti-CD3/CD28 antibodies for co-stimulation as described above