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Metabolites were separated using an Acquity UPLC BEH C8 1.7 m column (Waters, Milford, MA, USA) with mobile phase comprising 10 mmol/L ammonium acetate and 0.05% heptafluorobutyric acid in Milli-Q water (solution A) and 10 mmol/L ammonium acetate and 0.05% heptafluorobutyric acid (dissolved in 50 mL Milli-Q water) in methanol (solution B)

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Future interdisciplinary efforts, including in-depth mechanistic studies, large multicenter long-term follow-up trials, and translational research on precise intervention targets, are needed to address these gaps

Through his intensive athletic involvement, Dr