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This study was aimed to design stable and functional fusion proteins consisting of new protease-resistant GLP-1 mutants (mGLP1) genetically fused to DARPin as a critical step toward developing long-acting GLP-1 receptor agonists

The inhibition constant (Ki) was calculated from the binding energy (G) using the equation Ki = e G/RT where R represents the universal gas constant (1.985 10 3 kcal mol 1 K 1 ) and T is the temperature set at 298.15 K

By delivering these peptides directly to the nasal mucosa, this blend enables rapid absorption and bypasses the digestive system, potentially enhancing bioavailability and efficacy for laboratory purposes

doi: 10.1113/EP086587, 42