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Following incubation, cells were washed three times with ice-cold PBS and lysed by addition of 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl, 1% Triton X-100, pH 7.4)

Alternatively, miRNAs can repress translation by inducing premature ribosome detachment (Silvestro et al., 2019)

Because it's shown promise in protecting mitochondria - the tiny structures that serve as the energy-producing powerhouses of cells that weaken as the decades tick by - people have started to promote methylene blue as a way to slow cellular aging, including in the brain

2022;7(1):e001154

PMID: 19230461 Kanfer I et al (1993): Pharmacokinetics of oral decongestants