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Before applying to live cells, the cytotoxicity of TPE-DCV is evaluated by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay (Figure S13)

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The article points to individual variation in subcutaneous absorption rates and adipose tissue composition, and says people with higher baseline BMI or altered insulin sensitivity may take up the peptide differently across sites

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