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Hydrogen ion toxicity injures soil microbes that are intolerant to high amounts of H + ions [49]

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Although Amaranthus is generally considered a recalcitrant genus in this regard, several advances have been made to improve regeneration efficiency (Castellanos-Arvalo et al., 2020

Mice were anaesthetised with sodium pentobarbital (50 mg/kg saline) and transcardially perfused with ice-cold PBS 1X followed by RT PFA 4% for 5 min