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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature

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Finally, our last scenario reflects a quick de-escalation in which heightened rhetoric actually acts as a catalyst for renewed negotiations and a potential framework agreement that could result in some tariffs, but most likely at lower levels than initially proposed