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Any pigmented patch with these features needs urgent biopsy

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These advantages include allowing use during tidal (or normal) breathing, lack of need for timing and coordination, and less need for inspiratory effort during inhalation

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PCR amplification involved 30 cycles (94 C for 40s, 60 C for 40s, 72 C for 40s) after an initial 95 C/5min denaturation, followed by a final 72 C/5min extension, with products visualized on a 1% agarose gel and the recombinant vector subsequently digested with ECOR 1 and Xba 1 for sequencing (GENEray, China)