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To monitor cell viability, the cells were trypsinized, and apoptosis/necrosis induced by pneumococcal infection was evaluated using flow cytometry with an Annexin V/propidium iodide labeling kit (Invitrogen), showing 5% across all time points

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19 Poor sleep may aggravate rosacea by increasing stress and inflammatory signaling, and possibly disrupting skin microbiome balance and impairing skin barrier function

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