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Disruptions in brain insulin signaling can adversely affect the dopaminergic mesolimbic reward circuit, altering hedonic perceptions [89, 90]

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Intracellular and intrahepatic triglyceride (TG) measurement Intracellular TG and intrahepatic TG levels were measured using a TG assay kit (Applygen Technologies Inc., Beijing, China)

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2.7.2 Secondary outcome measures 2.7.2.1 Serological markers (SM) Serological markers will be monitored using liquid-phase suspension chip technology

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However, persistent or severe thirst warrants medical evaluation to rule out underlying conditions such as inadequately controlled diabetes or dehydration from gastrointestinal side effects

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